Hepatitis C virus Serology

Test Method
EIA
Specimen Required
Serum, Cadaver, Plasma
Spec Type Min
300ul
When Tested
Daily
Reference Ranges
Murex EIA (screening test)
cutoff formula;
neg control + 0.6
index formula; S/cutoff
index <0.9 = neg
index ³1.1 = pos
index 0.9-1.0 = equiv
Biorad EIA (supplemental test)
cutoff = pos control mean/5
neg <cutoff value
pos >cutoff value
equiv +/- 10% cutoff value
Units of Measurement
Optical density
Contact
9342 9647 / 9342 9648

Hepatitis C virus Serology – Screening

Test Method
EIA
Specimen Required
Blood (EDTA), Blood (Gel Tube), Blood (Lithium Heparin), Blood (Sodium Citrate), Blood (Whole), Plasma, Plasma (EDTA), Plasma (Lithium Heparin), Plasma (Sodium Citrate), Serum
Spec Type Min
300ul
When Tested
Daily
Turnaround Time
Up to 3 Days
Normal Ranges
Not Applicable
Units of Measurement
Optical density
Related Tests
Hepatitis C Virus Genotyping (HCVGEN1)
Contact
9342 9647 / 9342 9648

Hepatitis C virus Serology – Supplementary

Test Method
ECLIA
Specimen Required
Serum, Cadaver, Plasma
Spec Type Min
300ul
When Tested
3 x Weekly
Turnaround Time
Up to 5 Days
Reference Ranges
Reactive >= 1,Equiv 0.9-0.99, Non-Reactive < 0.9
Units of Measurement
COI
Contact
9342 9647 / 9342 9648

Hepatitis D virus PCR

Test Method
In-house quantitative RT-PCR; positive sample quantified by real-time PCR
Specimen Required
Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Plasma, Plasma (ACD), Plasma (EDTA), Serum
Spec Type Min
500 uL
When Tested
Weekly
Turnaround Time
2 Weeks
Reference Ranges
10 – 100,000,000 IU/ml
Cut Off Lower
10 IU/mL
Units of Measurement
IU/mL
Contact
9342 9615

Hepatitis D virus Serology (Ab)

Test Method
Antibody CLIA
Specimen Required
Blood (EDTA), Blood (Gel Tube), Blood (Lithium Heparin), Blood (Sodium Citrate), Blood (Whole), Plasma, Plasma (EDTA), Plasma (Lithium Heparin), Plasma (Sodium Citrate), Serum
Spec Type Min
300ul
When Tested
Up to 5 Days
Reference Ranges
Cut off = (0.5 x mean NC) + (0.5 x mean PC); =cut off = Reactive; > cut off = Non-Reavtive
Units of Measurement
Optical density
Contact
9342 9647 / 9342 9648

Hepatitis E virus Genotyping

Test Method
Conventional PCR; Capillary Sequencing
Specimen Required
Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Sodium Citrate), Blood (Whole), Plasma, Serum
Spec Type Min
500uL
When Tested
As requested
Turnaround Time
Three Weeks
Normal Ranges
Not Applicable
Reference Ranges
N/A
Cut Off Lower
1000 IU/ml
Units of Measurement
N/A
Contact
9342 9615

Hepatitis E virus PCR

Test Method
Commercial assay (Altona)
Specimen Required
Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Plasma, Serum
Spec Type Min
500 uL
When Tested
On request
Turnaround Time
N/A
Normal Ranges
Not Applicable
Reference Ranges
LLOQ 200 IU/mL
Cut Off Lower
200 IU/ml
Units of Measurement
IU/ml
Related Tests
Hepatitis E Virus Genotyping
Contact
9342 9615

Hepatitis E virus Serology

Test Method
IgG EIA
Specimen Required
Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Lithium Heparin), Blood (Sodium Citrate), Blood (Whole), Plasma, Plasma (ACD), Plasma (EDTA), Plasma (Lithium Heparin), Plasma (Sodium Citrate), Serum
Spec Type Min
100ul
When Tested
Weekly
Turnaround Time
Up to 5 Days
Reference Ranges
cutoff value = 0.5 + neg control mean
neg <cutoff value
pos >cutoff value
equiv +/- 10% of cutoff value
Units of Measurement
S/CO,
Contact
9342 9647 / 9342 9648

Herpes simplex virus (HSV) Phenotyping for drug resistance

Test Method
HSV PCR screen followed by Virus isolation & drug susceptibility
Specimen Required
Aspirate, Biopsy, Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Bone Marrow, Bowel Contents, Brushings, Cerebrospinal Fluid, Culture Isolate, Faeces, Fluid, Lavage, Milk, Non-human specimen, Nucleic Acid Extract, Other, Paraffin embedded tissue, Plasma, Plasma (ACD), Plasma (EDTA), Saliva, Scraping, Semen, Serum, Sputum, Swab, Tissue, Urine, Virus Isolate, Washings
Spec Type Min
200uL
When Tested
On request
Turnaround Time
>7 days
Reference Ranges
n/a
Related Tests
HSV drug resistance genotype
Billing Advice
Non-rebatable
Contact
9342 9628

Herpes simplex virus genotyping

Test Method
Conventional PCR
Specimen Required
Aspirate, Biopsy, Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Bone Marrow, Bowel Contents, Brushings, Cerebrospinal Fluid, Culture Isolate, Faeces, Fluid, Lavage, Milk, Non-human specimen, Nucleic Acid Extract, Other, Paraffin embedded tissue, Plasma, Plasma (ACD), Plasma (EDTA), Saliva, Scraping, Semen, Serum, Sputum, Swab, Tissue, Urine, Virus Isolate, Washings
Spec Type Min
200uL
When Tested
On request
Turnaround Time
7 Days
Reference Ranges
n/a
Billing Advice
Non-rebatable
Contact
9342 9628

Herpes simplex virus PCR(see General Herpes Multiplex PCR)

Test Method
Real-time TaqMan PCR
Specimen Required
Aspirate, Biopsy, Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Bone Marrow, Bowel Contents, Brushings, Cerebrospinal Fluid, Culture Isolate, Faeces, Fluid, Lavage, Milk, Non-human specimen, Nucleic Acid Extract, Other, Paraffin embedded tissue, Plasma, Plasma (ACD), Plasma (EDTA), Saliva, Scraping, Semen, Serum, Sputum, Swab, Tissue, Urine, Virus Isolate, Washings
Spec Type Min
200uL
When Tested
On request
Reference Ranges
3-45 Ct
Contact
9342 9628

Herpes simplex virus Serology (HSV) (IgG 1) Type Specific

Test Method
(HSV) (IgG 1) Type Specific
Specimen Required
Blood (Gel Tube), Blood (Whole), Plasma, Serum
Spec Type Min
300ul
When Tested
2x Weekly
Turnaround Time
Up to 7 Days
Reference Ranges
IgG & IgM
cutoff control value = 0.100
equiv = 0.1-0.2
low pos = 0.2-0.399
pos ³ 0.400
Batch specific
Type 1 & type 2
cutoff control mean = cutoff value
neg <cutoff value
pos > cutoff value
equiv +/- 10% of cutoff value
Units of Measurement
Optical density
Contact
9342 9647 / 9342 9648

Herpes simplex virus Serology (HSV) (IgG 2) Type Specific

Test Method
(HSV) (IgG 2) Type Specific
Specimen Required
Blood (Gel Tube), Blood (Whole), Plasma, Serum
Spec Type Min
300ul
When Tested
2x Weekly
Turnaround Time
Up to 7 Days
Reference Ranges
IgG & IgM
cutoff control value = 0.100
equiv = 0.1-0.2
low pos = 0.2-0.399
pos ³ 0.400
Batch specific
Type 1 & type 2
cutoff control mean = cutoff value
neg <cutoff value
pos > cutoff value
equiv +/- 10% of cutoff value
Units of Measurement
Optical density
Contact
9342 9647 / 9342 9648

Herpes simplex virus Serology (HSV) (IgG, IgM)

Test Method
(HSV) (IgG, IgM)
Specimen Required
Blood (EDTA), Blood (Gel Tube), Blood (Lithium Heparin), Blood (Sodium Citrate), Blood (Whole), Plasma, Plasma (EDTA), Plasma (Lithium Heparin), Plasma (Sodium Citrate), Serum
Spec Type Min
300ul
When Tested
2 x Weekly
Turnaround Time
Up to 7 Days
Units of Measurement
RLU
Contact
9342 9647 / 9342 9648

HIV 1/2 Confirmatory assay (supplemental) (Ab/Ag)

Test Method
Antibody / Antigen
Specimen Required
Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Lithium Heparin), Blood (Sodium Citrate), Blood (Whole), Plasma, Plasma (ACD), Plasma (EDTA), Plasma (Lithium Heparin), Plasma (Sodium Citrate), Serum, Cereberospinal Fluid
When Tested
On Request
Turnaround Time
Up to 5 Days
Contact
9342 9647 / 9342 9648

HIV-2 Drug Resistance Genotyping (Protease and RT regions)

Test Method
Conventional PCR , sequencing and analysis
Specimen Required
Plasma (ACD), Plasma (EDTA)
Spec Type Min
2ml of whole blood or 500ul of plasma
When Tested
On request
Turnaround Time
2-4 weeks
Reference Ranges
>500 copies/ml
Cut Off Lower
Sensitivity of assay is approximately 500 RNA copies/ml
Billing Advice
Non-rebatable
Contact
9342 9625

HIV-2 Proviral DNA Detection by Real Time PCR

Test Method
Real-time PCR (Taqman)
Specimen Required
Blood (ACD), Blood (EDTA), Blood (Whole)
Spec Type Min
2ml of whole blood (ACD/EDTA) or 200ul of buffy coat
When Tested
On request
Turnaround Time
10 days
Reference Ranges
validation in progress for limitations
Units of Measurement
Cycle threshold (Ct)
Billing Advice
Non-rebatable
Contact
9342 9625

Human herpesvirus 6 PCR

Test Method
Real-time TaqMan PCR
Specimen Required
Aspirate, Biopsy, Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Bone Marrow, Bowel Contents, Brushings, Cerebrospinal Fluid, Culture Isolate, Faeces, Fluid, Lavage, Milk, Non-human specimen, Nucleic Acid Extract, Other, Paraffin embedded tissue, Plasma, Plasma (ACD), Plasma (EDTA), Saliva, Scraping, Semen, Serum, Sputum, Swab, Tissue, Urine, Virus Isolate, Washings
Spec Type Min
200uL
When Tested
On request
Turnaround Time
same day / next day
Reference Ranges
3-45 Ct
Units of Measurement
Ct
Billing Advice
Non-rebatable
Contact
9342 9628

Human herpesvirus 6 Quantitation

Test Method
Real-time TaqMan PCR
Specimen Required
Aspirate, Biopsy, Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Bone Marrow, Bowel Contents, Brushings, Cerebrospinal Fluid, Culture Isolate, Faeces, Fluid, Lavage, Milk, Non-human specimen, Nucleic Acid Extract, Other, Paraffin embedded tissue, Plasma, Plasma (ACD), Plasma (EDTA), Saliva, Scraping, Semen, Serum, Sputum, Swab, Tissue, Urine, Virus Isolate, Washings
Spec Type Min
200uL
When Tested
On request
Turnaround Time
same day / next day
Reference Ranges
3-45 Ct
Units of Measurement
DNA Copies/mL
Billing Advice
Non-rebatable
Contact
9342 9628

Human herpesvirus 6 Serology

Test Method
IgG IFA, IgM IFA
Specimen Required
Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Lithium Heparin), Blood (Sodium Citrate), Blood (Whole), Plasma, Plasma (ACD), Plasma (EDTA), Plasma (Lithium Heparin), Plasma (Sodium Citrate), Serum
When Tested
Weekly
Turnaround Time
Up to 7 Days
Reference Ranges
IgG
titre >20 pos,
titre <20 neg,
titre 20 equiv;
IgM
titre <10 not detected,
titre >10 detected
Units of Measurement
TITRE
Contact
9342 9647 / 9342 9648

Human herpesvirus 7 PCR

Test Method
Real-time TaqMan PCR
Specimen Required
Aspirate, Biopsy, Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Sodium Citrate), Blood (Whole), Bone Marrow, Bowel Contents, Brushings, Cerebrospinal Fluid, Culture Isolate, Faeces, Fluid, Lavage, Milk, Nucleic Acid Extract, Paraffin embedded tissue, Plasma, Plasma (ACD), Plasma (EDTA), Saliva, Scraping, Semen, Serum, Sputum, Swab, Tissue, Urine, Virus Isolate, Washings
Spec Type Min
200uL
When Tested
On request
Turnaround Time
same day / next day
Reference Ranges
3-45 Ct
Units of Measurement
Ct
Billing Advice
Non-rebatable
Contact
9342 9628

Human herpesvirus 8 PCR

Test Method
Real-time TaqMan PCR
Specimen Required
Aspirate, Biopsy, Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Bone Marrow, Bowel Contents, Brushings, Cerebrospinal Fluid, Culture Isolate, Faeces, Fluid, Lavage, Milk, Non-human specimen, Nucleic Acid Extract, Other, Paraffin embedded tissue, Plasma, Plasma (ACD), Plasma (EDTA), Saliva, Scraping, Semen, Serum, Sputum, Swab, Tissue, Urine, Virus Isolate, Washings
Spec Type Min
200uL
When Tested
On request
Turnaround Time
same day / next day
Reference Ranges
3-45 Ct
Units of Measurement
Ct
Billing Advice
Non-rebatable
Contact
9342 9628

Human immunodeficiency virus – 1 proviral DNA tropism genotyping

Test Method
Conventional PCR, sequencing and analysis
Specimen Required
Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Plasma, Plasma (ACD), Plasma (EDTA), Serum
Spec Type Min
2ml of whole blood (ACD/EDTA) or 200ul of buffy coat
When Tested
On request
Turnaround Time
2-4 weeks
Reference Ranges
not detected (virus load)
Billing Advice
ViiV healthcare
Contact
9342 9625

Human immunodeficiency virus – 2 RNA Quantitation

Test Method
Real-time Taqman based PCR
Specimen Required
Plasma (ACD), Plasma (EDTA)
Spec Type Min
500ul of plasma or 2ml of whole blood
When Tested
On request
Turnaround Time
5 days
Reference Ranges
detectable from 500 copies/ml
Cut Off Lower
Target <500 copies/ml can be detected but not quantified
Units of Measurement
HIV-2 RNA copies/ml
Billing Advice
Medicare rebatable
Contact
9342 9625