Handbook Tests Archive | Page 4 of 10

Hepatitis B virus Serology – Core Antibody (Total)

Test Method: CLIA
Specimen Required: Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Lithium Heparin), Blood (Sodium Citrate), Blood (Whole), Plasma, Plasma (ACD), Plasma (EDTA), Plasma (Lithium Heparin), Plasma (Sodium Citrate), Serum
Spec Type Min: 500ul
Turnaround Time: Up to 3 Days
Reference Ranges: neg >= 1, pos < 0.9
Units of Measurement: Index
Contact: 9342 9647 / 9342 9648

Hepatitis B virus Serology – Core IgM Antibodies

Test Method: CLIA
Specimen Required: Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Lithium Heparin), Blood (Sodium Citrate), Blood (Whole), Plasma, Plasma (ACD), Plasma (EDTA), Plasma (Lithium Heparin), Plasma (Sodium Citrate), Serum
Spec Type Min: 500ul
Turnaround Time: Up to 5 Days
Reference Ranges: neg = 1
Units of Measurement: S/Co
Contact: 9342 9647 / 9342 9648

Hepatitis B virus Serology – Surface Antibody

Test Method: CLIA surface antibody
Specimen Required: Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Lithium Heparin), Blood (Sodium Citrate), Blood (Whole), Plasma, Plasma (ACD), Plasma (EDTA), Plasma (Lithium Heparin), Plasma (Sodium Citrate), Serum
Spec Type Min: 500ul
When Tested: See Notes
Turnaround Time: Up to 3 Days
Reference Ranges: neg = 11
Units of Measurement: IU/mL
Contact: 9342 9647 / 9342 9648

Hepatitis B virus Serology – Surface Antigen

Test Method: CLIA
Specimen Required: Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Lithium Heparin), Blood (Sodium Citrate), Blood (Whole), Plasma, Plasma (ACD), Plasma (EDTA), Plasma (Lithium Heparin), Plasma (Sodium Citrate), Serum
Spec Type Min: 500ul
When Tested: Daily
Turnaround Time: Up to 3 Days
Reference Ranges: Hep B sAg = neg = 0.05
Units of Measurement: IU/mL
Contact: 9342 9647 / 9342 9648

Hepatitis Be antigen detection

Test Method: Hbe antigen CLIA
Specimen Required: Serum or plasma (EDTA, ACD, heparin)
When Tested: Daily
Reference Ranges: 0.02 to 120 PEIU/ml HBeAg Neg = < 0.09 PEI U/ml, Equivocal = 0.09 - 0.11 PEI U/ml, Pos = 0.11 PEI U/ml.
Contact: 9342 9647 / 9342 9648

Hepatitis Be virus antibody detection

Test Method: Anti-Hbe test CLIA
Specimen Required: Serum or plasma (EDTA, ACD, heparin)
When Tested: Daily
Reference Ranges: Neg = 1.1, Equivocal 0.9 - 1.1, Pos <0.9
Contact: 9342 9647 / 9342 9648

Hepatitis C virus Genotyping

Test Method: Conventional RT-PCR and reverse-phase hybridization (LiPA)
Specimen Required: Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Plasma, Plasma (ACD), Plasma (EDTA), Serum
Spec Type Min: 500 uL
When Tested: 1 x weekly
Turnaround Time: Two weeks
Normal Ranges: Not Applicable
Reference Ranges: cut-off 1000 IU/ml
Cut Off Lower: 1000 IU/ml
Units of Measurement: Not Applicable
Related Tests: Hepatitis C Virus Genotyping (HCVGT2)
Contact: 9342 9615

Hepatitis C virus PCR

Test Method: Alinity M, COBAS 6800
Specimen Required: Blood (EDTA), Blood (Whole), Plasma, Serum
Spec Type Min: 700uL
When Tested: 3x Weekly
Turnaround Time: 6 days
Normal Ranges: N/A
Reference Ranges: 12 - 100,000,000 IU/mL
Cut Off Lower: 12 IU/mL
Units of Measurement: IU/ml
Contact: 9342 9615

Hepatitis C virus Quantification

Test Method: Alinity M
Specimen Required: Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Plasma, Plasma (ACD), Plasma (EDTA), Serum
Spec Type Min: 1 ml
When Tested: 3x Weekly
Turnaround Time: 6 Days.
Normal Ranges: N/A
Reference Ranges: 12 - 100,000,000 IU/mL
Cut Off Lower: 12 IU/mL
Units of Measurement: IU/ml
Contact: 9342 9615

Hepatitis C virus Serology

Test Method: EIA
Specimen Required: Serum, Cadaver, Plasma
Spec Type Min: 300ul
When Tested: Daily
Reference Ranges: Murex EIA (screening test)
cutoff formula;
neg control + 0.6
index formula; S/cutoff
index <0.9 = neg
index ³1.1 = pos
index 0.9-1.0 = equiv
Biorad EIA (supplemental test)
cutoff = pos control mean/5
neg <cutoff value
pos >cutoff value
equiv +/- 10% cutoff value
Units of Measurement: Optical density
Contact: 9342 9647 / 9342 9648

Hepatitis C virus Serology – Screening

Test Method: EIA
Specimen Required: Blood (EDTA), Blood (Gel Tube), Blood (Lithium Heparin), Blood (Sodium Citrate), Blood (Whole), Plasma, Plasma (EDTA), Plasma (Lithium Heparin), Plasma (Sodium Citrate), Serum
Spec Type Min: 300ul
When Tested: Daily
Turnaround Time: Up to 3 Days
Normal Ranges: Not Applicable
Units of Measurement: Optical density
Related Tests: Hepatitis C Virus Genotyping (HCVGEN1)
Contact: 9342 9647 / 9342 9648

Hepatitis C virus Serology – Supplementary

Test Method: ECLIA
Specimen Required: Serum, Cadaver, Plasma
Spec Type Min: 300ul
When Tested: 3 x Weekly
Turnaround Time: Up to 5 Days
Reference Ranges: Reactive >= 1,Equiv 0.9-0.99, Non-Reactive < 0.9
Units of Measurement: COI
Contact: 9342 9647 / 9342 9648

Hepatitis D virus PCR

Test Method: In-house quantitative RT-PCR; positive sample quantified by real-time PCR
Specimen Required: Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Plasma, Plasma (ACD), Plasma (EDTA), Serum
Spec Type Min: 500 uL
When Tested: Weekly
Turnaround Time: 2 Weeks
Reference Ranges: 10 – 100,000,000 IU/ml
Cut Off Lower: 10 IU/mL
Units of Measurement: IU/mL
Contact: 9342 9615

Hepatitis D virus Serology (Ab)

Test Method: Antibody CLIA
Specimen Required: Blood (EDTA), Blood (Gel Tube), Blood (Lithium Heparin), Blood (Sodium Citrate), Blood (Whole), Plasma, Plasma (EDTA), Plasma (Lithium Heparin), Plasma (Sodium Citrate), Serum
Spec Type Min: 300ul
When Tested: Up to 5 Days
Reference Ranges: Cut off = (0.5 x mean NC) + (0.5 x mean PC); =cut off = Reactive; > cut off = Non-Reavtive
Units of Measurement: Optical density
Contact: 9342 9647 / 9342 9648

Hepatitis E virus Genotyping

Test Method: Conventional PCR; Capillary Sequencing
Specimen Required: Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Sodium Citrate), Blood (Whole), Plasma, Serum
Spec Type Min: 500uL
When Tested: As requested
Turnaround Time: Three Weeks
Normal Ranges: Not Applicable
Reference Ranges: N/A
Cut Off Lower: 1000 IU/ml
Units of Measurement: N/A
Contact: 9342 9615

Hepatitis E virus PCR

Test Method: Commercial assay (Altona)
Specimen Required: Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Plasma, Serum
Spec Type Min: 500 uL
When Tested: On request
Turnaround Time: N/A
Normal Ranges: Not Applicable
Reference Ranges: LLOQ 200 IU/mL
Cut Off Lower: 200 IU/ml
Units of Measurement: IU/ml
Related Tests: Hepatitis E Virus Genotyping
Contact: 9342 9615

Hepatitis E virus Serology

Test Method: IgG EIA
Specimen Required: Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Lithium Heparin), Blood (Sodium Citrate), Blood (Whole), Plasma, Plasma (ACD), Plasma (EDTA), Plasma (Lithium Heparin), Plasma (Sodium Citrate), Serum
Spec Type Min: 100ul
When Tested: Weekly
Turnaround Time: Up to 5 Days
Reference Ranges: cutoff value = 0.5 + neg control mean
neg <cutoff value
pos >cutoff value
equiv +/- 10% of cutoff value
Units of Measurement: S/CO,
Contact: 9342 9647 / 9342 9648

Herpes simplex virus (HSV) Phenotyping for drug resistance

Test Method: HSV PCR screen followed by Virus isolation & drug susceptibility
Specimen Required: Aspirate, Biopsy, Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Bone Marrow, Bowel Contents, Brushings, Cerebrospinal Fluid, Culture Isolate, Faeces, Fluid, Lavage, Milk, Non-human specimen, Nucleic Acid Extract, Other, Paraffin embedded tissue, Plasma, Plasma (ACD), Plasma (EDTA), Saliva, Scraping, Semen, Serum, Sputum, Swab, Tissue, Urine, Virus Isolate, Washings
Spec Type Min: 200uL
When Tested: On request
Turnaround Time: >7 days
Reference Ranges: n/a
Related Tests: HSV drug resistance genotype
Billing Advice: Non-rebatable
Contact: 9342 9628

Herpes simplex virus genotyping

Test Method: Conventional PCR
Specimen Required: Aspirate, Biopsy, Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Bone Marrow, Bowel Contents, Brushings, Cerebrospinal Fluid, Culture Isolate, Faeces, Fluid, Lavage, Milk, Non-human specimen, Nucleic Acid Extract, Other, Paraffin embedded tissue, Plasma, Plasma (ACD), Plasma (EDTA), Saliva, Scraping, Semen, Serum, Sputum, Swab, Tissue, Urine, Virus Isolate, Washings
Spec Type Min: 200uL
When Tested: On request
Turnaround Time: 7 Days
Reference Ranges: n/a
Billing Advice: Non-rebatable
Contact: 9342 9628

Herpes simplex virus PCR(see General Herpes Multiplex PCR)

Test Method: Real-time TaqMan PCR
Specimen Required: Aspirate, Biopsy, Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Whole), Bone Marrow, Bowel Contents, Brushings, Cerebrospinal Fluid, Culture Isolate, Faeces, Fluid, Lavage, Milk, Non-human specimen, Nucleic Acid Extract, Other, Paraffin embedded tissue, Plasma, Plasma (ACD), Plasma (EDTA), Saliva, Scraping, Semen, Serum, Sputum, Swab, Tissue, Urine, Virus Isolate, Washings
Spec Type Min: 200uL
When Tested: On request
Reference Ranges: 3-45 Ct
Contact: 9342 9628

Herpes simplex virus Serology (HSV) (IgG 1) Type Specific

Test Method: (HSV) (IgG 1) Type Specific
Specimen Required: Blood (Gel Tube), Blood (Whole), Plasma, Serum
Spec Type Min: 300ul
When Tested: 2x Weekly
Turnaround Time: Up to 7 Days
Reference Ranges: IgG & IgM
cutoff control value = 0.100
equiv = 0.1-0.2
low pos = 0.2-0.399
pos ³ 0.400
Batch specific
Type 1 & type 2
cutoff control mean = cutoff value
neg <cutoff value
pos > cutoff value
equiv +/- 10% of cutoff value
Units of Measurement: Optical density
Contact: 9342 9647 / 9342 9648

Herpes simplex virus Serology (HSV) (IgG 2) Type Specific

Test Method: (HSV) (IgG 2) Type Specific
Specimen Required: Blood (Gel Tube), Blood (Whole), Plasma, Serum
Spec Type Min: 300ul
When Tested: 2x Weekly
Turnaround Time: Up to 7 Days
Reference Ranges: IgG & IgM
cutoff control value = 0.100
equiv = 0.1-0.2
low pos = 0.2-0.399
pos ³ 0.400
Batch specific
Type 1 & type 2
cutoff control mean = cutoff value
neg <cutoff value
pos > cutoff value
equiv +/- 10% of cutoff value
Units of Measurement: Optical density
Contact: 9342 9647 / 9342 9648

Herpes simplex virus Serology (HSV) (IgG, IgM)

Test Method: (HSV) (IgG, IgM)
Specimen Required: Blood (EDTA), Blood (Gel Tube), Blood (Lithium Heparin), Blood (Sodium Citrate), Blood (Whole), Plasma, Plasma (EDTA), Plasma (Lithium Heparin), Plasma (Sodium Citrate), Serum
Spec Type Min: 300ul
When Tested: 2 x Weekly
Turnaround Time: Up to 7 Days
Units of Measurement: RLU
Contact: 9342 9647 / 9342 9648

HIV 1/2 Confirmatory assay (supplemental) (Ab/Ag)

Test Method: Antibody / Antigen
Specimen Required: Blood (ACD), Blood (EDTA), Blood (Gel Tube), Blood (Lithium Heparin), Blood (Sodium Citrate), Blood (Whole), Plasma, Plasma (ACD), Plasma (EDTA), Plasma (Lithium Heparin), Plasma (Sodium Citrate), Serum, Cereberospinal Fluid
When Tested: On Request
Turnaround Time: Up to 5 Days
Contact: 9342 9647 / 9342 9648

HIV-1 Proviral DNA Drug Resistance Genotyping (protease, RT and integrase regions)

Test Method: Conventional PCR, sequencing and analysis
Specimen Required: Blood (ACD), Blood (EDTA), Blood (Whole)
Spec Type Min: 2mL of whole blood (ACD/EDTA) or 200uL buffy coat
When Tested: On Request
Turnaround Time: 2-4 Weeks
Cut Off Lower: Ct <36
Billing Advice: Non Rebateable
Contact: 9342 9625

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